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mouse wnt3a elisa kit  (Cusabio)


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    Cusabio mouse wnt3a elisa kit
    Mouse Wnt3a Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+wnt3a+elisa+kit/Mouse+Protein+Wnt-3a(WNT3A)+ELISA+kit/pmc07810654-167-9-15
    Average 92 stars, based on 5 article reviews
    mouse wnt3a elisa kit - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: HIFα regulates developmental myelination independent of autocrine Wnt signaling
    Article Snippet: .. The Wnt3a protein level was measured by the mouse Wnt3a ELISA kit (M13146365, CSB-EL026136MO, Cusabio) according to the manufacturer’s instruction. .. The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma).

    Article Title: HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling
    Article Snippet: .. The Wnt3a protein level was measured by the mouse Wnt3a ELISA kit (M13146365, CSB-EL026136MO, Cusabio) according to the manufacturer instruction. .. The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (catalog # {"type":"entrez-nucleotide","attrs":{"text":"LK003176","term_id":"635211093","term_text":"LK003176"}} LK003176 , Worthington; 250 U/ml; catalog #D5025, Sigma-Aldrich).

    Article Title: HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling
    Article Snippet: .. 154 155 Enzyme-linked immunosorbent assay (ELISA): The Wnt3a protein level was measured by 156 the mouse Wnt3a ELISA kit (M13146365, CSB-EL026136MO, Cusabio) according to the 157 manufacturer’s instruction. .. 158 159 Primary OPC culture and gene manipulation: The mouse cortex between P0 and P2 was 160 collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; 161 #D5025, Sigma).

    Article Title: HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling
    Article Snippet: .. ELISA The Wnt3a protein level was measured by the mouse Wnt3a ELISA kit (M13146365, CSB-EL026136MO, Cusabio) according to the manufacturer instruction. .. Primary OPC culture and gene manipulation The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (catalog # {"type":"entrez-nucleotide","attrs":{"text":"LK003176","term_id":"635211093","term_text":"LK003176"}} LK003176 , Worthington; 250 U/ml; catalog #D5025, Sigma-Aldrich).

    Article Title: HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling
    Article Snippet: .. The Wnt3a protein level was measured by the mouse Wnt3a ELISA kit (M13146365, CSB-EL026136MO, Cusabio) according to the manufacturer instruction. ..

    other:

    Article Title: Reduced Oligodendrocyte Precursor Cell Impairs Astrocytic Development in Early Life Stress
    Article Snippet: To examine the concentrations of Wnt3a, Wnt7a, and Wnt7b proteins in OPC/OL conditional medium and brain lysates, ELISA kits specific to the ligands (mouse Wnt3a Elisa Kit, CSB‐EL026136MO; mouse Wnt7a Elisa Kit, CSB‐EL026141MO; mouse Wnt7b Elisa Kit, CSB‐EL026142MO; CUSABIO) were used according to the manufacturer's instructions.



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    Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and <t>Wnt5a</t> protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.
    Anti Mouse Wnt3a Elisa Kit, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+wnt3a+elisa+kit/human+wnt5a+elisa+kit+okeh00723/pmc10187694-83-30-37
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    anti-mouse wnt3a elisa kit - by Bioz Stars, 2026-10
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    mouse wnt3a elisa kit  (Cusabio)
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    Cusabio mouse wnt3a elisa kit
    Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and <t>Wnt5a</t> protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.
    Mouse Wnt3a Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
    mouse wnt3a elisa kit - by Bioz Stars, 2026-10
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    wnt3a protein level  (Cusabio)
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    Cusabio wnt3a protein level
    Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and <t>Wnt5a</t> protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.
    Wnt3a Protein Level, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    csb el026136mo  (Cusabio)
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    Cusabio csb el026136mo
    Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and <t>Wnt5a</t> protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.
    Csb El026136mo, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and Wnt5a protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.

    Journal: Aging and Disease

    Article Title: Osteoblastic Wls Ablation Protects Mice from Total Body Irradiation-Induced Impairments in Hematopoiesis and Bone Marrow Microenvironment

    doi: 10.14336/AD.2022.1026

    Figure Lengend Snippet: Deletion of Wls from Col1a1 -expressing cells does not affect Wls expression or the secretion and senescence of HSCs in the spleens of TBI-exposed mice. Four-week-old Wls fl/fl and Col-Cre;Wls fl/fl mice were exposed to sub-lethal TBI, and (A) splenic Wls mRNA and (B) Wnt3a and Wnt5a protein levels in spleen supernatants were determined 4 weeks post-TBI by qRT-PCR and ELISA, respectively (n = 6). At the same time post-TBI, (C) the number of HSCs and (D) MitoSox- or (E) C 12 FDG-positive HSCs in the spleens of those mice were determined by flow cytometry (n = 5). Col1a1-Cre activity was determined in (F) the trabecular and cortical zones of the femur and (G) the spleens of mutant and control mice 4 weeks post-TBI. The representative images in panel (F) show regions stained with X-gal (blue), which indicate the active Cre recombinase sites in the sections. Here, the images exhibiting the X-gal-specific intensity at average level among five different samples were represented. The p values in panels A and B were determined by unpaired Student’s t -test. The p values in panels C-E were calculated using unpaired non-parametric Wilcoxon t -test. ns, not significant.

    Article Snippet: The protein levels of Wnt3a, Wnt5a, and DKK1 in BM and spleen lysates isolated from TBI-exposed Wls fl/fl and Col-Cre ; Wls fl/fl mice were quantified using anti-mouse Wnt3a (OKEH03470), Wnt5a (OKEH04420), and DKK1 (OKEH04073) ELISA kits (Aviva Systems Biology, San Diego, CA, USA), respectively, at various times (0-72 h) after TBI exposure according to the manufacturer’s instructions.

    Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Activity Assay, Mutagenesis, Control, Staining

    TBI acutely reduces the numbers of peripheral WBCs and lymphocytes but not the BM levels of Wnt ligands, and that is ameliorated by osteoblastic Wls depletion. The levels of circulating (A) WBCs, (B) lymphocytes, (C) granulocytes, (D) RBCs, and (E) platelets in the mouse groups were measured using an automated complete blood cell counter at the indicated times (h) after TBI (n = 5). (F) The BM levels of Wnt3a and Wnt5a in Wls fl/fl and Col-Cre;Wls fl/fl mice were evaluated by IHC when they were 4 weeks of age. Representative data from five different samples are shown. Scale bars = 100 μm. (G) The area (%) positively stained with Wnt3a or Wnt5a in the IHC assay was calculated (n = 5). (H) Levels of Wnt3a and Wnt5a mRNA in whole BM lysate were determined by qRT-PCR (n = 5). Protein levels of (I) Wnt3a and (J) Wnt5a in the whole BM lysate were determined by ELISA at the indicated times after TBI (n = 5). The p values in all panels were determined by non-parametric Wilcoxon t -test. The superscripts a-c indicate significant differences among the groups compared with the value of non-TBI control or mutant group by ANOVA. The symbol ‘*’ in panels I and J indicate significant difference at p < 0.043 by the Wilcoxon t -test. ns, not significant.

    Journal: Aging and Disease

    Article Title: Osteoblastic Wls Ablation Protects Mice from Total Body Irradiation-Induced Impairments in Hematopoiesis and Bone Marrow Microenvironment

    doi: 10.14336/AD.2022.1026

    Figure Lengend Snippet: TBI acutely reduces the numbers of peripheral WBCs and lymphocytes but not the BM levels of Wnt ligands, and that is ameliorated by osteoblastic Wls depletion. The levels of circulating (A) WBCs, (B) lymphocytes, (C) granulocytes, (D) RBCs, and (E) platelets in the mouse groups were measured using an automated complete blood cell counter at the indicated times (h) after TBI (n = 5). (F) The BM levels of Wnt3a and Wnt5a in Wls fl/fl and Col-Cre;Wls fl/fl mice were evaluated by IHC when they were 4 weeks of age. Representative data from five different samples are shown. Scale bars = 100 μm. (G) The area (%) positively stained with Wnt3a or Wnt5a in the IHC assay was calculated (n = 5). (H) Levels of Wnt3a and Wnt5a mRNA in whole BM lysate were determined by qRT-PCR (n = 5). Protein levels of (I) Wnt3a and (J) Wnt5a in the whole BM lysate were determined by ELISA at the indicated times after TBI (n = 5). The p values in all panels were determined by non-parametric Wilcoxon t -test. The superscripts a-c indicate significant differences among the groups compared with the value of non-TBI control or mutant group by ANOVA. The symbol ‘*’ in panels I and J indicate significant difference at p < 0.043 by the Wilcoxon t -test. ns, not significant.

    Article Snippet: The protein levels of Wnt3a, Wnt5a, and DKK1 in BM and spleen lysates isolated from TBI-exposed Wls fl/fl and Col-Cre ; Wls fl/fl mice were quantified using anti-mouse Wnt3a (OKEH03470), Wnt5a (OKEH04420), and DKK1 (OKEH04073) ELISA kits (Aviva Systems Biology, San Diego, CA, USA), respectively, at various times (0-72 h) after TBI exposure according to the manufacturer’s instructions.

    Techniques: Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Mutagenesis